The Antioxidant Activity of Atomized Extracts of the Leaves and Stems of Cnidoscolus diacanthus (Pax& K. Hoffm.) J.F. Macbr. from Peru and Their Effect on Sex Hormone Levels in Rats

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dc.contributor.authorTinco Jayo, Johnny Aldo
dc.contributor.authorEnciso Roca, Edwin Carlos
dc.date.accessioned2025-03-08T02:07:28Z
dc.date.available2025-03-08T02:07:28Z
dc.date.issued2024
dc.description.abstractIn this research, we aimed to determine the antioxidant activity of an atomized extract of Cnidoscolus diacanthus (Pax & K. Hoffm.) J.F. Macbr., known in Peru as “huanarpo hembra”, and its effect on sex hormone levels. Its phytochemical profile was determined using liquid chromatography–mass spectrometry (LC–MS), while its total phenol content (TPC) and total flavonoids (TFs) were determined using the Folin-Ciocalteu method and the aluminum chloride method. Its antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), the radical 2,2-azino-bis-3-ethylbenzthiazolin-6 sulfonic acid (ABTS), and ferric-reducing antioxidant power (FRAP). The biological activity of C. diacanthus and its effect on sexual hormones were determined in Holtzman rats of both sexes. Phytochemical analysis revealed the presence of flavonoids and phenolic compounds in its leaves and stems, mainly rutin, quercetin, chlorogenic acid, and genistein. However, the stem extract contained higher total phenol (464.38± 4.40 GAE/g) and flavonoid (369.17± 3.16 mg QE/g of extract) contents than the leaf extract (212.38± 3.19 mg GAE/g and 121.49± 2.69 mg QE/g). For DPPH, ABTS, and FRAP, the Trolox-equivalent antioxidant capacity (TEAC) was 597.20 ± 5.40 µmol/g, 452.67 ± 5.76 µmol/g, and 535.91 ± 1.56 µmol/g, respectively, for the stems, while for the leaves, it was 462.39± 3.99 µmol/g, 202.32± 5.20 µmol/g, and 198.13± 1.44 µmol/g, respectively. In terms of the values for hormonal levels, at a dose of 100 mg/kg of the extract, testosterone levels of 1.430 ng/mL (with the leaf extract) and 1.433 ng/mL (with the stem extract), respectively, were found in the male rats. Regarding estradiol levels, in the female rats, these were 10.425 ng/mL (leaf extract) and 8.775 ng/mL (stem extract), while their levels of luteinizing hormone were 0.320 mIU/mL (leaf extract) and 0.273 mIU/mL (stem extract). For the follicle-stimulating hormone, levels of 0.858 mIU/mL (leaf extract) and 0.840 mIU/mL (stem extract) were found in the female rats, and levels of 0.220 mIU/mL (leaf extract) and 0.200 mIU/mL (stem extract) were found in the male rats. It is concluded that the C. diacanthus stem extract had a greater antioxidant capacity than the leaf extract, while both extracts had a superior effect on the sex hormone levels in the female rats compared to the male rats.es_PE
dc.description.uriArtículo Científicoes_PE
dc.formatapplication/pdf
dc.identifier.otherArtículo Científico 11_Tin
dc.identifier.urihttps://repositorio.unsch.edu.pe/handle/20.500.14612/7539
dc.language.isoenes_PE
dc.publisherUniversidad Nacional de San Cristóbal de Huamangaes_PE
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.sourceUniversidad Nacional de San Cristóbal de Huamangaes_PE
dc.sourceRepositorio Institucional - UNSCHes_PE
dc.subjectHuanarpo hembraes_PE
dc.subjectSexual hormoneses_PE
dc.subjectFertility plantses_PE
dc.subjectPeruvian plantses_PE
dc.subjectEthnopharmacologyes_PE
dc.subjectAntioxidant agentses_PE
dc.subjectFecundityes_PE
dc.titleThe Antioxidant Activity of Atomized Extracts of the Leaves and Stems of Cnidoscolus diacanthus (Pax& K. Hoffm.) J.F. Macbr. from Peru and Their Effect on Sex Hormone Levels in Ratses_PE
dc.typeinfo:eu-repo/semantics/articleen_US
renati.advisor.orcidhttps://orcid.org/0000-0002-8970-9379
renati.advisor.orcidhttps://orcid.org/0000-0003-4089-5905
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