ARTÍCULOS CIENTÍFICOS DE DOCENTES INVESTIGADORES

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Browsing ARTÍCULOS CIENTÍFICOS DE DOCENTES INVESTIGADORES by Author "Peña Rojas, Gilmar"

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    Dodonaea viscosa Jacq. Induces cytotoxicity, antiproliferative activity, and cell death in colorectal cancer cells via regulation of caspase 3 and p53
    (Universidad Nacional de San Cristóbal de Huamanga, 2023) Peña Rojas, Gilmar; Andía Ayme, Vidalina
    Colorectal cancer (CRC) is the third most common cancer diagnosed worldwide and is the second leading cause of cancer-related death due to an insuf?ciency prognosis and is generally diagnosed in the last step of development. The Peruvian ?ora has a wide variety of medicinal plants with therapeutic potential in several diseases. Dodonaea viscosa Jacq. is a plant used to treat in?ammatoryprocessaswellas gastrointestinal diseases. The aim of this study was to examine the cytotoxic, antiproliferative, and cell death-inducing effects of D. viscosa on colorectal cancer cells (SW480 and SW620). The hydroethanolic extract was obtained by maceration at 70% ethanol, the phytochemical constituents were identi?ed by LC-ESI-MS.D. viscosa revealed 57 compounds some of them are: isorhamnetin, kaempferol, quercetin, methyl dodovisate B, hardwickiic acid, viscosol, and dodonic acid. Regarding the antitumoral activity, D. viscosa induced cytotoxic and antiproliferative activity in both SW480 and SW620 cancer cells, accompanied with, important changes in mitochondrial membrane potential, formation of the Sub G0/G1 population and increasing levels of apoptotic biomarkers (caspase 3 and the tumor suppressor protein p53) in the metastatic derivative cell line (SW620), suggesting an intrinsic apoptotic process after the treatment with the hydroethanolic extract of D. viscosa.
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    Efecto de la suplementación con bacterias ácido-lácticas sobre los parámetros productivos en cuyes (Cavia porcellus) de engorde, Ayacucho - Perú
    (Universidad Nacional de San Cristóbal de Huamanga, 2023) Quijano Pacheco, Wilber Samuel; Andía Ayme, Vidalina; Peña Rojas, Gilmar
    Con el objetivo de evaluar la suplementación con bacterias ácido-lácticas en las raciones de cuyes (Cavia porcellus) de engorde sobre los parámetros productivos, se planteó la experiencia bajo el diseño estadístico completo al azar con arreglo factorial (3T x 3D): tratamientos: T1 (Lactobacillus casei), T2 (L. acidophilus) y T3 (mezcla de L. casei y L. acidophilus) y, tres dosis: 0, 50 y 100 ml (10? UFC ml??) que se ofreció mezclando al alimento. En todos los casos con tres repeticiones y 3 cuyes como unidad experimental, para la comparación de medias se utilizó la prueba de contraste de Tukey, se experimentó con 108 cuyes machos con dos semanas de nacidos. El tiempo de evaluación fue 54 días. Los resultados para consumo de alimento e índice de conversión alimenticia fueron no significativos (p<0,05). Sin embargo, para el peso vivo y ganancia de peso estadísticamente fueron significativos y con la prueba de Tukey la mejor dosis fue de 50 ml, así como para el porcentaje de carcasa. Para la retribución económica el costo de alimento fue similar para todos los tratamientos y dosis. No obstante, numéricamente el uso de los microorganismos favorece para la obtención de cuyes con mejores pesos comerciales para la venta.
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    Effect of Naphthaleneacetic Acid (NAA) and 6-Benzylamino Purine (BAP) on In-Vitro Propagation of "Mashua" (Tropaeolum tuberosum Ruíz & Pavón) Morphotypes from Peru
    (Universidad Nacional de San Cristóbal de Huamanga, 2023) Andía Ayme, Vidalina; Peña Rojas, Gilmar; Pereda Medina, Alex
    Background: Tropaeolum tuberosum Ru?z & Pavón “mashua” is a native species of the central Andes and different propagation methods in vitro have been evaluated. However, this research has studied the in vitro propagation of 15 mashua (Tropaeolum tuberosum Ruíz & Pavón) morphotypes from the Ayacucho and Apurímac regions (Peru) Objective: To evaluate the effect of the additives naphthaleneacetic acid (NAA) and 6-benzylamino purine (BAP) on the micro-propagation rate of T. tuberosum. Material and Methods: For in vitro establishment, seedlings were used after disinfection with sodium hypochlorite, propagated in Murashige and Skoog (MS) medium. For the evaluation of the effect of NAA and BAP additives, nodes were isolated from in vitro seedlings after 30 days of cultivation and transferred to MS medium supplemented with 3% sucrose, pH 5.6 and 7 g/L of agar according to T1 (MS + NAA 1ppm) and T2 (MS + BAP 1ppm) treatments. The samples were cultivated for 28 days at 19 °C ± 2 °C with photoperiods of 16 h/light and 8 h/darkness. Results: After evaluating 20 repetitions for each of the three treatments, the addition of NAA or BAP does not improve the micropropagation rate of practically all the morphotypes studied. Furthermore, BAP behaved as an inhibitor of the development of mashua seedlings. Conclusion: The best micropropagation medium of Tropaeolum tuberosum Ruíz & Pavón (mashua) is the basic Murashige and Skoog (MS) medium without the NAA or BAP additives.
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    Improved Production of Mashua (Tropaeolum tuberosum) Microtubers MAC-3 Morphotype in Liquid Medium Using Temporary Immersion System (TIS-RITA®)
    (Universidad Nacional de San Cristóbal de Huamanga, 2022) Peña Rojas, Gilmar; Andía Ayme, Vidalina; Carhuaz Condori, Roxana
    Essential molecules are embedded within the millenary crop Tropaeolum tuberosum (mashua); these compounds are critical for the Andean people’s traditional diet and extensively utilized by the pharmaceutical industry in Peru. In the Andean region, conventional cropping techniques generate microtubers susceptible to a viral infection, which substantially endangers mashua’s production. Therefore, we developed an innovative in vitro technique condition for enhancing the agriculture process for micro tubers production. The temporary immersion system (TIS) permits the production of high-quality microtubers in a reduced space, a lower amount of time, and in large quantities compared with tubers grown under traditional conditions. To obtain T. tuberosum’s microtubers via TIS, we propagated seedlings, utilizing TIS-RITA® vessels. A set of immersion frequency times were evaluated. Interestingly, results showed that immersion at 2 min every 3 h was more bene?cial compared with 2 min every 5 h based on microtubers produced after 10 weeks from the treatments, revealing an ef?cient frequency setting which outputted improved microtubers quality and production.
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    In silico evaluation of Dodonic Acid from Dodonaea viscosa Jacq on target Proteins from Staphylococcus aureus
    (Universidad Nacional de San Cristóbal de Huamanga, 2022) Andía Ayme, Vidalina; Peña Rojas, Gilmar
    Dodonaea viscosa Jacq is known as “chamana” in the popular flora of Peru. the traditional medicine uses its leaves as ingredient in fermented beverages from Zea mays and also in external uses for antiinflammatory diseases. The aim was to study the role of dodonic acid against several protein targets of S. aureus. this study was focused on to analyse the role of dodonic acid against S. aureus target proteins such as on sortase-A, DNA gyrase, dihydrofolate reductase (DhFR), clumping factor, dehydrosqualene synthase, and undecaprenyl di-phosphate synthase as a promising candidate molecule. the docking analysis of dodonic acid showed the best docking score energy on S. aureus undecaprenyl diphosphate synthase with -11.2 kcal/mol and demonstrated to be a very stable molecule at physiological conditions during the molecular dynamic for 50 ns. As conclusion, the extract demonstrated to be active against S. aureus and dodonic acid might be a promising molecule acting on the S. aureus undecaprenyl diphosphate synthase.
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    Microrganismos acido lácticos y levaduras como suplemento en las raciones para el engorde de cuyes (Cavia porcellus) Ayacucho, Perú
    (Universidad Nacional de San Cristóbal de Huamanga, 2022) Quijano Pacheco, Wilber Samuel; Andía Ayme, Vidalina; Peña Rojas, Gilmar
    Los microorganismos ácido lácticos poseen un efecto benéfico en el engorde de los cuyes, al mejorar la degradación de la materia orgánica y prevenir la salmonelosis. El objetivo fue evaluar los microrganismos acido lácticos y levaduras como suplemento en las raciones para el engorde de cuyes (Cavia porcellus) en Ayacucho. Se activaron las bacterias en caldo lactosado a 37°C por 48 horas en anaerobiosis, a través de diluciones consecutivas se sembró en agar Rogosa sharpe incubándose a 37°C por 24 a 48 horas para recuento del número de células vivas/ml. Se utilizaron 20 gazapos machos destetados con tamaño y peso homogéneos. Se tuvo 5 tratamientos suplementando con bacterias ácido lácticas al alimento balanceado: T1 (Lactobacillus acidophilus), T2 (Lactobacillus casei), T3 (Lactobacillus acidophilus y Lactobacillus casei) y T5 (testigo), brindándose a libre discreción y alfalfa al 10% de su peso vivo más agua. Como unidad experimental se planteó bajo el diseño completamente randomizado con 5 tratamientos, 2 repeticiones y 2 cuyes. Los resultados mostraron que para el peso vivo y la ganancia de peso resultaron no significativos (p<0.05) y para el consumo de alimento y la conversión alimenticia fueron significativas. Por la prueba de Tukey resultó que los tratamientos con la combinación T3 Lactobacillus acidophilus + Lactobacillus casei y T2 Lactobacillus casei fueron mejores lográndose peso comercial para el mercado en ocho semanas de tratamiento, además mejores rendimientos de carcasa. Con ello se puede intuir que los microorganismos influyen positivamente en el desarrollo de los cuyes.